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KMID : 0358419970400010170
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Korean Journal of Obstetrics and Gynecology
1997 Volume.40 No. 1 p.170 ~ p.180
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DNA Ploidy Heterogeneity in Primary and Metastatic Lesion of Epithelial Ovarian Cancer
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김종혁
남주현/허주령/김용만/김영탁/목정은
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Abstract
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Tumor DNA content measured by flow cytometry may be a predictor in the prognosis of epithelial ovarian cancer, but the results have been inconsistent. It is recognized that these conflicting results are at least partly due to the variation of DNA
content between the samples from the same patient(i.e., intratumoral DNA heterogeneity). The purposes of this retrospective study were to investigate the frequency and the nature of DNA heterogeneity in epithelial ovarian cancer and to evaluate
the
prognostic significance of DNA heterogeneity itself. Thirty-two patients with stage ⅡtoⅣ epithelial ovarian cancer who were managed at Asan Medical Center between May 1993 and April 1996 were analysed. Measurements of the nuclear DNA content
were
performed on samples from primary and metastatic lesion using paraffin embedded archival tissues by Epics(Coulter Inc.) flow cytometry. In two cases, the metastatic tumor was minute and did not reveal a separable peak on repeated examination. DNA
heterogeneity was defined as different ploidy pattern or difference of the DNA indices than 0.15 between primary and metastatic tumors. DNA heterogeneity was fund in 11 cases(36.7%), and the number of cases with homogeneous diploid and that with
homogeneous aneuploid tumor were 5(16.7%) and 14(46.7%) respectively. In evaluation of prognostic significance of DNA heterogeneity using correlation with serum CA 125 level after second course of chemotherapy and residual tumor size after
cytoreductive
surgery among these three groups, the patients with DNA heterogeneity were considered to show intermediate prognosis between those with homogeneous diploid and homogeneous aneuploid tumor. In conclusion, DNA heterogeneity in epithelial ovarian
cancer is
considerable in frequency and may have prognostic value.
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KEYWORD
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